Library construction:
We provide full Library construction and sequencing service for NGS, starting at whatever stage required, according to customer’s needs.
When planning a new NGS project we suggest researchers to start with consulting with us. Our team together with a bioinformatics advisor has a reputation of providing expert, personalized service from the first stages of experimental design, choice of library preparation method and sequencing parameters.
Our experienced stuff have years of experience with most of the commercial kits available on the market. Some commercial kits are in standard usage and in stock in our lab and can be ordered in any numbers upon request. Other commercial kits can be ordered by customers and sent to our lab together with the samples for efficient and reliable handling. Service for homemade protocols is also available, if a detailed manual is provided.
Our in-house stock Libraries:
TruSeq kit of Illumina
https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-rna-v2.html
Instruction for samples delivery:
The minimal RNA amount for library generation is 100ng dilute in 52ul.
The maximal RNA amount for library generation is 2ug dilute in 52ul.
Samples should be delivered in strips only marked in chronological numbers.
In the submission form , fill the table with your sample name respectively to the chronological numbers marked on the strip.
Sense kit of lexogen
https://www.lexogen.com/sense-mrna-sequencing/
Instruction for samples delivery:
The minimal RNA amount for library generation is 500ng dilute in 52ul.
The maximal RNA amount for library generation is 2ug dilute in 52ul.
Samples should be delivered in strips only marked in chronological numbers.
In the submission form , fill the table with your sample name respectively to the chronological numbers marked on the strip.
NEBNext Multiplex Small RNA Library prep kit of New England
Instruction for samples delivery:
The minimal RNA amount for library generation is 100ng dilute in 7ul.
The maximal RNA amount for library generation is 1ug dilute in 7ul.
Samples should be delivered in strips only marked in chronological numbers.
In the submission formלצרף לינק לטופס הגשת דוגמאות , fill the table with your sample name respectively to the chronological numbers marked on the strip.
Nextera kit of Illumina
https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/nextera-dna.html
Instruction for samples delivery:
Samples should be 1-10ng dilute in 20ul.
Samples should be delivered in strips only, marked in chronological numbers.
In the submission formלצרף לינק לטופס הגשת דוגמאות , fill the table with your sample’s name according to its chronological numbers as marked on the strip.